| 摘要: |
| 为选育出霉酚酸（mycophenolic acid， MPA）发酵生产的优良菌株，以产霉酚酸的短密青霉菌（Penicillium brevicompactum）S-225菌株作为原始菌株，利用甲基磺酸乙酯（ethyl methanesulfonate， EMS）、紫外诱变（ultraviolet， UV）和常压室温等离子体诱变（atmospheric room temperature plasma， ARTP）叠加诱变的方法，选育出1株遗传稳定、且对Cu2+耐受性强的菌株SA-2，其10 t中试霉酚酸发酵效价达到8 458 mg/L，比原始菌株提高了137.3%，且其200 t发酵生产效价达到了8 769 mg/L。该研究结果对霉酚酸工业化发酵生产具有重要意义。 |
| 关键词: 霉酚酸 高产菌株 诱变 选育 |
| DOI：10.14188/j.ajsh.2022.04.009 |
| 分类号:Q939.97 |
| 基金项目: |
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| Screening of Penicillium brevicompactum SA-2 with high yield of mycophenolic acid |
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ZHANG Ping, SHI Yanpeng, NIU Chun, LI Chunling
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Ningxia Tairui Pharmaceutical Company Limited， Yinchuan 750101， Ningxia， China
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| Abstract: |
| In order to select a good strain for mycophenolic acid fermentation， Penicillium brevicompactum S-225 strain producing mycophenolic acid was used as the original strain， and treated by superimposed mutagenesis of ethyl methanesulfonate （EMS）， ultraviolet （UV） and atmospheric room temperature plasma （ARTP）. After mutagenesis， SA-2 strain with good genetic stability and high Cu2+ tolerance was screened out. Its mycophenolic acid fermentation potency was up to 8458 mg/L， which was 137.3% higher than that of the original strain. The results are of great significance to the industrial fermentation of mycophenolic acid. |
| Key words: mycophenolic acid high yield strain mutagenesis screening |
