| 摘要: |
| 嗜盐古菌是一类应用前景广阔的微生物资源，但由于缺乏高效灵活的遗传操作工具，限制了这类生物在科学研究和工业生产方面的应用。CRISPR-Cas系统存在于绝大多数古菌中，应用其内源性CRISPR-Cas系统进行遗传操作是一种兼具可行性和简便性的策略。近年来，人们对Ⅰ-B亚型CRISPR-Cas系统的研究已从模式古菌沃氏富盐菌（Haloferax volcanii）拓展到其他嗜盐古菌中。本文总结了Ⅰ-B亚型CRISPR-Cas系统的分子机制，并介绍了利用这种内源性CRISPR-Cas系统在嗜盐古菌中进行遗传操作的研究进展。 |
| 关键词: 嗜盐古菌 CRISPR-Cas系统 Ⅰ-B亚型 |
| DOI：10.14188/j.ajsh.2021.05.002 |
| 分类号:Q93 |
| 基金项目:国家自然科学基金（31970028） |
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| Recent progress on molecular mechanisms and application of the type Ⅰ-B CRISPR-Cas system in haloarchaea |
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ZHANG Jia, WU Yi, TANG Bing, TANG Xiaofeng
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College of Life Sciences， Wuhan University， Wuhan 430072， Hubei， China
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| Abstract: |
| Haloarchaea are a kind of biological resources with broad application prospects. However， due to the lack of efficient and flexible genetic manipulation tools， the application of these organisms in scientific research and industrial production is restricted. CRISPR-Cas system is present in the majority of archaea. Therefore， harnessing their endogenous CRISPR-Cas machineries for genetic manipulation is a feasible and convenient strategy. In recent years， research on the type Ⅰ-B CRISPR-Cas system has expanded from the type archaeon Haloferax volcanii to other haloarchaea. In this paper， we summarize the recent progress on the molecular mechanisms of the type Ⅰ-B CRISPR-Cas system， which has been successfully used for genetic manipulation in some haloarchaea. |
| Key words: haloarchaeon CRISPR-Cas system type Ⅰ-B |
