| 摘要: |
| 为系统地探索甘肃巴丹吉林沙漠土壤中细菌多样性并评估其潜在的应用价值，采集6个代表性样点的沙漠土壤，运用高通量测序技术评估沙漠土壤细菌的群落结构和预测功能特征，同时选用5种培养基对其中细菌进行分离培养，并对代表性的菌株进行酶活性筛选。免培养结果显示，甘肃巴丹吉林沙漠土壤中的细菌分属于30门70纲155目227科343属，这些微生物主要参与碳氮硫等元素循环和有机物降解，此外还检测到1.5%的未知微生物。通过纯培养方法共获得368株细菌，归类于4门20目37科64属，其中24株属于潜在新分类单元，菌株中18.3%有淀粉酶活性，51.7%产蛋白酶以及55.5%有酯酶活性。结果表明甘肃巴丹吉林沙漠土壤中蕴藏着多样性较高、功能酶丰富的微生物资源，具有重要的研究意义和应用价值。 |
| 关键词: 巴丹吉林沙漠 扩增子 分离培养 功能预测 生物多样性 功能酶 |
| DOI：10.14188/j.ajsh.2021.02.006 |
| 分类号:Q938.1 |
| 基金项目:国家科技部及教育部“111计划”[国科发专(2019)475]；贵州省第六批人才基地项目[RCJD2018-22]；贵州省科技厅社会发展攻关支撑项目[黔科合支撑(2019)2873]；贵州省科学技术厅国际科技合作基地[黔科合平台人才(2020)4101]；贵州省科技厅优秀青年科技人才培养计划项目［黔科合(20175639)］；贵州省科技计划项目[黔科合支撑(2020)4Y220] |
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| Biodiversity and characteristics of enzyme producing bacteria from Badain Jaran Desert in Gansu Province |
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KAN Yu1,2, LI Shuai3, HU Chaojian3, LI Wenjun3, HUANG Jin2,4, ZHAO Liang1,2, DONG Yuhua2, KANG Yinqian1,2
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1.Department of Microbiology， School of Basic Medicine， Guizhou Medical University， Guiyang 550025， Guizhou， China;2.Research Talent Base for the Relationship between Microbiology and Human Health in Guizhou Province and Key Laboratory of Pathogen Biology in Universities in Guizhou Province， Guiyang 550025， Guizhou， China;3.School of Life Science， Sun Yat-sen University，Guangzhou 510275，Guangdong， China;4.Department of Biochemistry and Molecular Biology， School of Basic Medicine， Guizhou Medical University， Guiyang 550025， Guizhou， China
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| Abstract: |
| In order to explore the diversity and enzyme of bacteria systematically from Badain Jaran Desert in Gansu Province， 6 representative soil samples were collected. The community structure and functional prediction of the bacteria in these samples were evaluated by high-throughput sequencing technology. At the same time， the bacteria were further isolated and cultivated by using 5 kinds of media， and the representative strains were screened for the ability of producing amylase， protease and esterase. Results from culture-independent method showed that the bacteria were annotated into 30 phyla， 70 classes， 155 orders， 227 families and 343 genera. The predicted functions were related mainly to carbon， nitrogen and sulfur cycles and organic degradation. In addition， there were 1.5% of unidentified microbes. Through the comparative analysis based on 16S rRNA gene sequence similarities， a total of 368 strains were obtained. These strains were affiliated to 64 genera of 37 families in 20 orders within 4 phyla， of which 24 strains were potential new taxa. Of these strains， 18.3% produced amylase， 51.7% produced protease and 55.5% produced esterase. These results demonstrate that there are abundant bacteria and large number of functional strains in the Badan Jaran Desert in Gansu Province， which has important research significance and application value. |
| Key words: Badan Jaran Desert amplicon isolation function prediction biodiversity enzyme function |
