| 摘要: |
| β-葡萄糖苷酶是一种糖苷水解酶，广泛存在于动物、植物和微生物中。β-葡萄糖苷酶能够水解非还原性末端糖基，在植物细胞壁代谢、植物激素激活以及逆境防御等方面发挥着重要作用。β-葡萄糖苷酶依据其氨基酸序列可以分为GH1、GH3、GH5、GH7、GH9、GH12、GH35、GH116等8个家族；但是，目前仅对GH1和GH3有较深入的研究，其他家族的功能依旧不清楚。综述了近年来植物中β-葡萄糖苷酶的结构、理化性质、底物特异性、催化机制以及糖苷水解酶家族在植物中的功能等方面的研究进展，总结了植物中β-葡萄糖苷酶研究中存在的问题，并指出今后的研究方向。 |
| 关键词: β-葡萄糖苷酶 基因家族 活性位点 异源表达 |
| DOI：10.14188/j.ajsh.2021.02.001 |
| 分类号:Q946.5 |
| 基金项目:国家自然科学基金（31470388） |
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| Research progress of plant β-glucosidase |
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WANG Chen, LI Jiaru
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Sate Key Laboratory of Hybrid Rice， College of Life Sciences，Wuhan University，Wuhan 430072，Hubei，China
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| Abstract: |
| β-glucosidase is a glycoside hydrolase， widely found in animals， plants and microorganisms. β-glucosidase can hydrolyze non-reducing terminal glucosides in plants， and thus it plays an important role in plant cell wall metabolism， phytohormone activation and defense response. β-glucosidase can be divided into GH1， GH3， GH5， GH7， GH9， GH12， GH35， GH116 based on amino acid sequence. However， only GH1 and GH3 have been studied deeply， and the functions of other families are still unclear. The research progress on the structure， physicochemical properties， substrate specificity， catalytic mechanism and the function of β-glucosidase in plants in recent years are reviewed in this paper. Problems in the research are summarized and the future research direction is pointed out. |
| Key words: β-glucosidase gene family active site heterologous expression |
