| 摘要: |
| 灰肉红菇（Russula griseocarnosa）是岭南地区著名的野生食药用真菌。本研究通过组织分离的方法，从灰肉红菇子实体分离获得一株内生真菌，经形态学和分子生物学分析鉴定为爪哇虫草（Cordyceps javanica）。以牛津杯抑菌圈法及二倍稀释法测试了其菌丝体胞内和胞外多糖粗提物对金黄色葡萄球菌（Staphylococcus aureus）和铜绿假单胞菌（Pseudomonas aeruginosa）的抑菌活性。结果表明：水溶性胞内、胞外多糖对金黄色葡萄球菌及铜绿假单胞菌均具有抑菌活性，最低抑菌浓度（MIC）为37.5 mg/mL。 |
| 关键词: 爪哇虫草 抑菌活性 多糖 灰肉红菇 |
| DOI：10.14188/j.ajsh.2020.05.013 |
| 分类号:S646.9 |
| 基金项目:广东省科技计划项目（2019B121202005）；广东扬帆计划引进创新创业团队项目（2017YT05S115） |
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| Isolation， identification and its antibacterial activity of an endophytic fungus of Russula griseocarnosa |
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WU Xiaoxian1, LIU Yuanchao1,2, WANG Ao1, ZHUO Lijun1, HU Huiping1,3
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1.State Key Laboratory of Applied Microbiology Southern China/Guangdong Provincial Key Laboratory of Microbial Safety and Health， Guangdong Institute of Microbiology， Guangzhou 510070， Guangdong， China;2.School of Biolagy and Biological Engineering， South China University of Technology， Guangzhou 510006， Guangdong， China;3.Jiaoling Tiehan Big Health Industry Investment Co. Ltd.， Jiaoling 514100， Guangdong， China
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| Abstract: |
| Russula griseocarnosa is a well-known wild edible and medicinal fungus in Lingnan area. An endophytic fungus strain， which was identified as Cordyceps javanica by morphology and molecular biology， was isolated from the fruiting body of Russula griseocarnosa by tissue isolation method. We detected the antimicrobial activity of intracellular and extracellular polysaccharides extracted from the mycelia of the endophyte against Staphylococcus aureus and Pseudomonas aeruginosa respectively by the Oxford Cup method. Their minimum inhibitory concentration （MIC） was also tested by the double dilution method. The results showed that both the water-soluble intracellular and extracellular polysaccharides had antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa， with a minimum inhibitory concentration （MIC） of 37.5 mg/mL. |
| Key words: Cordyceps javanica antimicrobial activity polysaccharide Russula griseocarnosa |
