| 摘要: |
| 大肠杆菌的蛋白质表达平台在工业和农业中得到了广泛应用，使目的蛋白质表达后释放至胞外更有利于大规模的生产。目前，已经研究出许多改善外源蛋白胞外含量的方法。本文从蛋白质分泌机制、菌株、信号肽、载体和培养条件的选择和优化改造、密码子的优化和蛋白质跨膜转运过程的改善等方面总结了提高大肠杆菌表达外源蛋白的胞外含量的各种策略，指出多因素协同作用才能更全面地提升蛋白质的胞外产量。 |
| 关键词: 大肠杆菌 外源蛋白 胞外含量 |
| DOI：10.14188/j.ajsh.2020.04.002 |
| 分类号:Q935 |
| 基金项目:国家自然科学基金面上项目（31470185） |
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| Strategies for increasing extracellular content of heterologous protein in Escherichia coli |
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CAO Lei1, TANG Xiaofeng1
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College of Life Sciences, Wuhan University, Wuhan 430072, Hubei, China
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| Abstract: |
| In recent years, the protein expression platform of Escherichia coli has been widely used in industry and agriculture. It is more conducive for large-scale production to produce target protein into the extracellular medium. Many methods have been developed to improve the extracellular content of heterologous protein. This paper summarizes the strategies for increasing extracellular content of heterologous protein in E. coli such as protein secretion mechanisms, selection and optimization of hosts, signal peptides, vectors and culture conditions, improvements of codons and protein transmembrane transports. The synergy of multiple factors could increase protein extracellular production more comprehensively. |
| Key words: E. coli heterologous protein extracellular content |
