| 摘要: |
| 研究脱氢弯孢酶菌素(Dehydrocurvularin, DCV)对脂多糖（lipopolysaccharide,LPS）诱导的小鼠腹腔（peritoneal macrophages, PMs）和骨髓来源的巨噬细胞（bone-marrow-derived macrophages, BMDMs）的影响。MTT方法筛选最佳药物浓度处理不同来源的巨噬细胞，采用Western blot方法检测诱导型一氧化氮合酶（induceble nitric oxide synthase, iNOS）、环氧化酶-2(cyclooxygenase-2, COX-2)及炎性信号通路NF-κB和MAPK的相关蛋白的表达。结果显示，脱氢弯孢酶菌素可显著抑制LPS诱导的iNOS、COX-2的表达，且呈剂量依赖性。NF-κB和MAPK信号通路相关的蛋白激活也受到了明显的抑制。结果表明，脱氢弯孢酶菌素可显著抑制LPS诱导的不同来源的免疫细胞的炎症。 |
| 关键词: 脱氢弯孢霉菌素 炎症 NF-κB通路 MAPK通路 |
| DOI：10.14188/j.ajsh.2020.02.013 |
| 分类号:R285.5 |
| 基金项目:湖北省教育厅科研计划项目（D20182101） |
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| LPS-simulated inflammatory response by dehydrocurvularin in macrophages |
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ZHONG Jing1, Zhao qun2, Li Yulan3, Yu Xianjun1,2, DENG Zhangshuang1
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1.College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, Hubei, China;2.Department of biochemistry, Faculty of Basic Medicine, Hubei University of Medicine, Shiyan 442000, Hubei, China;3.Yichang Central People’s Hospital, the First Clinical Medical College of China Three Gorges University, Yichang 443003, Hubei, China
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| Abstract: |
| To study the effect of dehydrocurvularin (DCV) in lipopolysaccharide (LPS)-induced mouse peritoneal macrophages (PMs) and bone-derived macrophages (bone-marrow-derived macrophages, BMDMs). MTT was used to screen the optimal drug concentration to treat macrophages from different sources. Western-blot method was used to detect cyclooxygenase-2 (COX-2) and inflammatory signaling pathway NF-κB and MAPK related proteins. DCV significantly inhibited LPS-induced COX-2 expression in a dose-dependent manner. Protein activation associated with NF-κB and MAPK signaling pathways was also significantly inhibited. DCV inhibits LPS-induced inflammatory properties of immune cells from different sources. |
| Key words: dehydrocurvularin inflammation NF-κB pathway MAPK pathway |
