| 摘要: |
| 以燕麦品种“定燕2号”和“晋燕14号”为研究材料，研究紫外线B（ultraviolet rays-B， UV-B）辐射增强条件下，一氧化氮（nitric oxide， NO）供体硝普钠（sodium nitroprusside， SNP）对燕麦幼苗活性氧含量、膜脂过氧化和抗氧化物质含量的影响。结果表明：NO能够降低UV-B辐射增强条件下“定燕2号”和“晋燕14号”的超氧阴离子（superoxide radical， O2·-）产生速率、羟自由基（hydroxyl free radical， OH·）、过氧化氢（hydrogen peroxide， H2O2）和丙二醛（malondialdehyde， MDA）含量。UV-B辐射时间为6 h，施加SNP时，“定燕2号”的超氧化物歧化酶（superoxide dismutase， SOD）活性和过氧化物酶（peroxidase， POD）活性、“晋燕14号”的SOD活性高于其他处理组；UV-B辐射时间为3 h，施加SNP时，“定燕2号”、“晋燕14号”的抗坏血酸过氧化物酶（ascorbate peroxidase， APX）活性显著高于0 h、12 h处理组。UV-B辐射时间为6 h，“定燕2号”的脱氢抗坏血酸（dehydroascorbic acid， DHA）含量显著高于其他处理组；而UV-B辐射时间为0 h，“晋燕14号”的DHA 含量显著高于其他处理组。NO对UV-B辐射增强下“定燕2号”和“晋燕14号”抗氧化性影响的综合测评均为：3 h>6 h>12 h>0 h。本研究结果为NO在燕麦育种、抗氧化机制等提供理论依据。 |
| 关键词: 燕麦 UV-B辐射 抗氧化系统 |
| DOI：10.14188/j.ajsh.2019.03.010 |
| 分类号: |
| 基金项目:吕梁市科技攻关重点研发项目 NYZDYF2017-67-1;吕梁市特色植物功能成分工程技术研究中心 GCJSYJZX2017-67-3;山西省重点研发计划项目 201803D221011-6;山西省重点实验室项目 201805D111012吕梁市科技攻关重点研发项目（NYZDYF2017-67-1），吕梁市特色植物功能成分工程技术研究中心（GCJSYJZX2017-67-3），山西省重点研发计划项目（201803D221011-6），山西省重点实验室项目（201805D111012） |
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| Nitric oxide alleviates the oxidative damage of oat seedlings under UV-B radiation enhancement |
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DU Jingqi1,MA Xiaoming1,WANG Donghui1
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Department of Life Sciences， Lü liang University， Lü liang 033000， Shanxi， China
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| Abstract: |
| The effects of NO on the content of reactive oxygen, membrane lipid peroxidation and antioxidant substances in oat seedlings under UV-B radiation enhancement were studied using oat varieties Dingyan 2 and Jinyan 14. The results showed that NO reduced the rate of O2·- production, content of OH·, H2O2 and MDA in both oat varieties under the condition of UV-B radiation enhancement. The SOD activity and POD activity of Dingyan 2 and SOD activity of Jinyan 14 were higher than those of other treatment groups when the duration of UV-B radiation was 6h and SNP was applied. The APX activity of Dingyan 2 and Jinyan 14 was significantly higher than that of 0h and 12 h when the duration of UV-B radiation was 3 h and SNP was applied. The DHA content of Dingyan 2 was significantly higher than that of other treatment groups when the duration of UV-B radiation was 6 h. The DHA content of Jinyan 14 was significantly higher than that of other treatment groups when the duration of UV-B radiation was 0 h. The comprehensive evaluation of the effect of NO on the antioxidant activity of both Dingyan 2 and Jinyan 14 under UV-B radiation enhancement was as follows: 3 h>6 h>12 h>0 h. The results of this study provide theoretical basis for NO in breeding and antioxidant mechanism. |
| Key words: oat UV-B radiation antioxidant system |
