| 摘要: |
| 骆驼刺（Alhagi sparsifolia Shap.）是新疆民间常用药用植物。为了解骆驼刺内生菌的多样性，获得骆驼刺的内生菌资源，本实验在新疆维吾尔克拉玛依盐碱戈壁（北纬45°16’，东经85°2’）采集骆驼刺。利用常规平板分离方法进行植物内生菌菌株的分离、培养，测定菌株16S rDNA 基因序列，并结合系统发育分析进行鉴定。从骆驼刺中共分离到可培养内生菌50株，分属于葡萄球菌属（Staphylococcus）、芽胞杆菌属（Bacillus）、芽胞八叠球菌属（Sporosarcina）、微杆菌属（Exiguobacterium）、气球菌属（Aerococcus）、巨型球菌属（Macrococcus）、多米杆菌（Domibacillus）、巴尔加瓦菌属（Bhargavaea）、微球菌属（Micrococcus）、棒杆菌属（Corynebacterium）、考克菌属（Kocuria）、细杆菌属（Microbacterium）、副球菌属（Paracoccus）、马西利亚菌属（Massilia）和耐辐射球菌属（Deinococcus）15个菌属。其中葡萄球菌属占绝对优势，其次为芽胞杆菌属,为环境和植物当中广泛存在的菌属，分离获得的其他细菌与骆驼刺生长环境（盐渍化严重，高辐射）有关，内生菌多样性与骆驼刺在新疆干旱、寒冷、盐碱土壤环境中的适应性机制具有密切的联系。 |
| 关键词: 盐碱戈壁 骆驼刺 植物内生菌 群落多样性 |
| DOI：10.14188/j.ajsh.2019.01.008 |
| 分类号:Q9 |
| 基金项目:新疆维吾尔自治区自然科学基金编号: 2017D07C212新疆维吾尔自治区自然科学基金(项目编号: 2017D07C212） |
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| Isolation and identification of endophyte from Alhagi sparsifolia Shap. in Xinjiang Gobi desert area |
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ZHANG Xiaobo1,MA Yan2,XIONG Wenjuan2,ZHENG Qian2,WEI Jie1,CHEN Feng2,LIU Suhui2,SUN Yuping2
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1.School of Public Health, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;2.College of Basic Medical, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
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| Abstract: |
| Alhagi sparsifolia Shap. is a commonly used medicinal plant in Xinjiang. To understand the diversity of endophytic bacteria of Alhagi sparsifolia Shap., and to get some microorganism resources and promote the development and utilization of microbial resources of plant endogenous bacteria and their important functional genes in the future, the samples from desert in Xinjiang (karamay north latitude 45°16’, longitude 85°2’) were collected to isolate the endophytes. Morphological observation and 16S rDNA region sequence determination and phylogenetic tree were studied. 50 endophyte strains were obtained, and belonged to Staphylococcus, Bacillus, Sporosarcina, Exiguobacterium, Aerococcus, Macrococcus, Domibacillus, Bhargavaea, Micrococcus, Corynebacterium, Kocuria, Microbacterium, Paracoccus, Massilia, and Deinococcus. Among them, the genus Staphylococcus was the dominate group, followed by the genus Bacillus, which are widely existed in the environment and plant. The other isolated bacteria were related to the growth environment of camel spines (serious salinization and high radiation). It is found that the endophyte diversity is closely related to the adaptability mechanism of the plant in the arid, cold, saline alkali soil environment of Xinjiang. |
| Key words: Gobi desert environment Alhagi sparsifolia plant endogenous bacterium community diversity |
