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| 小花清风藤的化学成分及其对肝细胞的保护作用 |
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李曼姝1,李建桥1,胡建忠2,温秀风2,刘志成1,彭玉丹1,刘呈雄1,汪鋆植1,刘朝霞1
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1.三峡大学 生物与制药学院 天然产物研究与利用湖北省重点实验室;2.水利部 水土保持植物开发管理中心，北京 100038
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| 摘要: |
| 采用正相硅胶柱色谱、Sephadex LH-20柱色谱、重结晶等多种分离/纯化方法，利用核磁共振（NMR）波谱分析法，对小花清风藤茎叶的化学成分进行分离与鉴定。结果从小花清风藤茎叶的石油醚萃取部位分离并鉴定出4个化合物，包括2个五环三萜类化合物和2个其他类化合物，分别为24-羟基-3-氧代-12-齐墩果烯-28-酸(化合物1)，β-谷甾醇(化合物2)，豆甾醇(化合物3)和齐墩果酸(化合物4)。其中，化合物1为首次从该属植物中分离得到。采用四唑盐（MTT）法测试石油醚萃取物和分离得到的4个化合物对CCl4损伤的LO2肝细胞的保护作用，结果均显示出良好的肝细胞保护作用，其中石油醚部位和化合物4肝细胞保护作用最明显，能够显著提高CCl4损伤LO2肝细胞的存活率。 |
| 关键词: 小花清风藤 五环三萜 分离纯化 结构鉴定 保肝护肝 |
| DOI：10.14188/j.ajsh.2018.06.002 |
| 分类号:R284.1 |
| 基金项目:水利部水土保持植物开发管理中心合作项目(No. 2017-zg-kj-4/SDHZ2017015；2018-zg-kj-03)；国家自然科学基金青年科学基金项目（81803383） |
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LI Manshu1,LI Jianqiao1,HU Jianzhong2,WEN Xiufeng2,LIU Zhicheng1,PENG Yudan1,LIU Chengxiong1,WANG Junzhi1,LIU Zhaoxia1
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1.Hubei Key Laboratory of Natural Products Research and Development, College of Biological and Pharmaceutical Sciences, China Three Gorges University, Yichang 443002, Hubei, China;2.Soil and Water Conservation Centre, the Ministry of Water Resources, Beijing 100038, China
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| Abstract: |
| Various means such as silica gel, Sephadex LH-20, and recrystallization were used for separation and purification of chemical constituents from the stems and leaves of Sabia parviflora, and their structures were identified by nuclear magnetic resonance (NMR) spectra data. Four compounds were obtained from the petroleum ether extract of Sabia parviflora, including two pentacyclic triterpenes and two other compounds, and their structures were identified as 24-hydroxy-3-oxoolean-12-en-28-oic acid (1), β-sitosterol (2), stigmasterol (3) and oleanolic acid (4), respectively. Compound 1 was isolated from Sabia parviflora for the first time. Methyl thagolyl tetragoliam (MTT) assay was used to determine the protective effects of petroleum ether extract and four compounds on hepatocyte injury induced by CCl4 in LO2 cells. The results showed that all samples had hepatocyte protection activities. Among them, the petroleum ether extract and compound 4 had better hepatocyte protection activities, and they could significantly improve the survival rate of LO2 hepatocytes injured by CCl4. |
| Key words: Sabia parviflora pentacyclic triterpenoid separation and purification structural identification hepatocyte protection activity |
