| 摘要: |
| 本实验为研究番杏(Tetragonia tetragonioides)功能基因表达模式提供内参基因，根据登录在NCBI上植物的Actin基因的保守区域设计简并性引物，通过RT-PCR克隆获得番杏的Actin基因片段，将该片段连接于载体pGEM-T后进行测序，并将该基因序列通过生物信息学软件进行分析。结果显示，克隆所得基因片段大小为598 bp，编码198个氨基酸；该序列与登录在NCBI上的其他植物的Actin基因的核苷酸序列的同源性最大可达86%以上，编码蛋白的氨基酸序列同源性在88%以上。结果表明，本研究克隆所得的基因序列为番杏Actin基因片段。该基因命名为TtActin1，在GenBank的登录号为MH33308。 |
| 关键词: 番杏 肌动蛋白 序列分析 |
| DOI：10.14188/j.ajsh.2018.05.004 |
| 分类号: |
| 基金项目:国家重点研发计划项目(2016YFC1403002)；中国科学院A类战略性先导科技专项(XDA13020500)；“十二五”农村领域国家科技计划项目(2015BAL04B04) |
|
| Cloning and bioinformatics analysis of Actin gene fragment from Tetragonia tetragonioides |
|
YE Yuyan,LIANG Haifeng,YANG Lixiang
|
|
College of Life Sciences, Guangzhou University, Guangzhou 510006, Guangdong, China
|
| Abstract: |
| In order to provide reference gene for studying the expression pattern of functional genes in Tetragonia tetragonioides, the cDNA fragment of Actin from Tetragonia tetragonioides was amplified by RT-PCR, by adopting degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was analyzed by online bioinformatics software. Result showed that this Actin cDNA includes 598 base pairs, encoding a partial actin with 198 amino acids. Sequence alignments showed that it shares over 86% nucleotide sequence identity and 88%amino acid sequence identity with other plant actins. This study indicates that a new Actin cDNA fragment is isolated from Tetragonia tetragonioides. This cDNA is named TtActin1 and submitted to GenBank(accession number: MH33308). |
| Key words: Tetragonia tetragonioides actin sequence analysis |
