| 摘要: |
| 以大枣为原料，采用乙醇浸提法提取芦丁，以芦丁得率为评价指标，通过正交试验优化影响大枣芦丁得率的若干因素：液料比、乙醇浓度、浸提时间、浸提温度，确定其最佳工艺条件为液料比20∶1（mL∶g）、乙醇浓度60%、浸提时间3.0 h、浸提温度70 ℃，此条件下大枣芦丁得率为2.16%；大枣芦丁粗提物中芦丁含量为8.57%。对芦丁进行体外抗氧化能力测定结果表明，大枣芦丁具有一定的清除DPPH自由基的能力，5 μg/mL的大枣芦丁对DPPH自由基的清除率可达到58.80%， 25 μg/mL 的芦丁对OH自由基的清除率达49.80%，本研究对芦丁资源的进一步开发利用提供参考。 |
| 关键词: 大枣 芦丁 乙醇浸提 抗氧化性 |
| DOI：10.14188/j.ajsh.2018.04.012 |
| 分类号:S-3 |
| 基金项目:陕西省教育厅重点基金项目（17JS033）； 渭南师范学院特色学科建设项目18TSXK04 |
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| Extraction of rutin from jujube by ethanol and its antioxidant effect |
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LIU Nannan1, JIANG Shuixing2
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1.College of Chemistry and Material Science， Weinan Normal University， Weinan 714099， Shaanxi， China;2.Rizhao Jinsheng Biochemical Group Co.， Ltd， Rizhao 276800， Shandong， China
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| Abstract: |
| The present work discusses the rutin extraction by ethanol from jujube. With rutin yield as the evaluation index， the factors influencing the yield of jujube rutin such as liquid-solid ratio， ethanol concentration， extraction time and extraction temperature were optimized. The best process conditions were liquid to solid ratio 20∶1 （mL∶g）， ethanol concentration 60%， extraction time 3.0 h， and extraction temperature 70 ℃. Under these conditions， the yield of jujube rutin was 2.16%， and the content of rutin in jujube rutin crude extract was 8.57%. Rutin was tested for its antioxidant capacity in vitro. The results showed that jujube rutin had a certain ability to remove DPPH free radicals， 5 μg/mL jujube rutin could remove 58.80% of DPPH free radicals， and 25 μg/mL jujube rutin could remove 49.80% of OH free radicals. This study provides references for further development and utilization of rutin resources. |
| Key words: jujube rutin ethanol extraction antioxidant property |
