| 摘要: |
| 利用模式生物秀丽隐杆线虫,考察8种人体必需氨基酸对衰老的影响。首先建立秀丽隐杆线虫寿命模型,以雷帕霉素为阳性对照药,分别考察8种必需氨基酸对线虫生存时间的影响。再用筛选出的氨基酸处理线虫21d,通过秀丽隐杆线虫-绿脓杆菌感染模型,考察氨基酸对线虫的抗感染能力的影响,利用实时荧光定量Real-Time RT-PCR方法检测氨基酸处理线虫后DAF-16/FOXO下游基因和免疫相关基因的表达水平。结果表明8种必需氨基酸中苏氨酸和异亮氨酸既能延长野生型线虫的寿命又能延长daf-16突变型线虫的寿命,同时还能增强秀丽隐杆线虫抗绿脓杆菌感染的能力,并提高免疫相关基因lys-7、clec-67的表达水平,而DAF-16/FOXO下游基因表达没有明显变化。因此苏氨酸和异亮氨酸能延长线虫寿命、提高抗感染能力,且对线虫寿命的延长作用不完全依赖于DAF-16/FOXO转录因子。 |
| 关键词: 必需氨基酸 抗衰老 秀丽隐杆线虫 |
| DOI： |
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| Anti-aging effects of essential amino acids on Caenorhabditis elegans |
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BAI Zhihui,WANG Yan,ZHANG Rili,XU Weiheng,ZHANG Junping,School of Pharmacy,Second Military Medical University
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School of Pharmacy,Second Military Medical University
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| Abstract: |
| Using the model organismCaenorhabditis elegans to investigate the effects of 8human essential amino acids on aging was studied.First,we established the C.eleganslifespan model and observed the survival time to investigate the effects of 8essential amino acids on aging of C.elegans.Rapamycin was used as a positive control drug.Then,C.elegans was treated with the selected amino acids for 21 days.Using the C.elegans-Pseudomonas aeruginosa infection model,we observed the effects of amino acids on anti-infective ability of C.elegans.The expression of genes regulated by DAF-16/FOXO and immune-related genes in C.elegans were detected using real-time RT-PCR.The results showed that threonine/isoleucine prolonged the lifespan of C.elegans wild-type and daf-16 mutant.Each of the two amino acids also enhanced the anti-infective ability of C.elegans against P.aeruginosa.Moreover,threonine/isoleucine increased the expression of immune-related genes lys-7 and clec-67,while genes down-regulated by DAF-16/FOXO were unaffected.Therefore,threonine and isoleucine can prolong the lifespan and enhance the anti-infective ability of C.elegans,and their effects on lifespan do not completely depend on DAF-16/FOXO transcription factor. |
| Key words: essential amino acid anti-aging Caenorhabditis elegans |
