| 摘要: |
| 为了研究木瓜多糖的提取、分离、纯化与抗氧化活性,采用水提醇沉法提取皱皮木瓜中的多糖,得多糖Ⅰ;利用Sevag法除去多糖中的蛋白质后得多糖Ⅱ;以30%H_2O_2脱除色素后再次醇沉得到精制多糖Ⅲ;透析除去小分子后利用AB-8大孔树脂进行分离以水、30%、50%、70%和95%乙醇洗脱,其中水洗脱部分多糖为Ⅳ。用苯酚-硫酸法测定多糖含量。多糖Ⅰ得率为9.83%,多糖含量(纯度,下同)为64.45%;脱蛋白后多糖Ⅱ中多糖含量为78.23%;经脱色后多糖Ⅲ含量达88.39%;大孔树脂水洗脱部分多糖Ⅳ含量为89.74%。以DPPH(2,2-二苯基-1-苦肼基)清除率和Fe~(3+)还原力方法测定木瓜多糖的抗氧化活性,木瓜多糖均体现出一定的抗氧化作用,呈浓度依赖性增强,其中多糖Ⅰ、Ⅱ表现出更好的作用。 |
| 关键词: 皱皮木瓜多糖 Sevag法 大孔树脂分离 抗氧化活性 |
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| Isolation,purification,and antioxidant activity of polysaccharides from Chaenomeles speciosa Nakai |
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YAO Chengcheng,ZHANG Xucheng,YE Chao,DENG Zhangshuang,LIU Zhaoxia,Hubei Key Laboratory of Natural Products Research,Development,College of Biological,Pharmaceutical Sciences,Three Gorges University
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Hubei Key Laboratory of Natural Products Research and Development,College of Biological and Pharmaceutical Sciences,Three Gorges University
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| Abstract: |
| This paper is aimed to study the extraction,separation,purification,and antioxidant capacity of polysaccharides fromChaenomeles speciosa.PolysaccharideⅠ was obtained by water extraction and alcohol precipitation with the yield of 9.83%.Then,proteins were removed by using Sevag method to obtain polysaccharideⅡ.Furtherly,30% H_2O_2 was used to remove the pigments to get more refined polysaccharideⅢ.AB-8macroporous resin was used to obtain the water,30%,50%,70%,95%ethanol eluting fraction,of which the water fraction was named as polysaccharideⅣ.The contents of polysaccharidesⅠ,Ⅱ,Ⅲ andⅣ were determined as 64.45%,78.23%,88.39%and 89.74%,respectively.It was found that these polysaccharides showed antioxidant activities by DPPH(2,2-diphenyl-1-picrylhydrazyl)clearance and Fe~(3+)reducing power assays,and polysaccharidesⅠandⅡ showed better antioxidant activity. |
| Key words: Chaenomeles speciosa polysaccharide Sevag method macroporous resin separation antioxidant activity |
