| 摘要: |
| 选择新疆阿魏植物内生真菌中一株药理活性较强的菌,研究其生长特性,分别考察不同培养基、温度、光照及起始pH值4个单因素对菌种生长的影响,通过响应面试验优化,确定菌种最佳生长条件。结果表明,在黑暗条件下,采用土豆培养基(PDA),pH值为7,温度25℃环境中,该菌种可正常生长和繁衍,菌株平均生长速率为10.25mm·d~(-1)。 |
| 关键词: 新疆阿魏 内生真菌 生长特性 响应面 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Research on Growth Characteristics of An Endophytic Fungus of Endangered Plant Ferula sinkiangensis K M Shen |
|
SONG Jun,ZHU Jun,ZHAO Yaqin,LI Xiaojin,College of Traditional Chinese Medicine,Xinjiang Medical University
|
|
College of Traditional Chinese Medicine,Xinjiang Medical University,Xinjiang Institute of Chinese Traditional Medicine and Ethnic Medicine
|
| Abstract: |
| Growth characteristics of an endophytic fungus of endangered plant Ferula sinkiangensis K.M.Shen were studied in order to lay a foundation for further research and development.Effects of culture media,temperature,light and initial pH value on the growth of the strain were investigated.The optimal growth conditions were determined by response surface experiment.The results showed that under the dark conditions,using the potato culture medium(PDA),the fungus could grow at a average rate of 10.25 mm·d~(-1) when pH was 7and temperature was 25℃. |
| Key words: Ferula sinkiangensis K M Shen endophytic fungi growth characteristics response surface |
