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| 厚藤Actin基因片段的克隆及序列分析 |
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郭艳1,2,张美1,2,夏快飞1,2,简曙光1,2,陈建通1,2
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1. 中国科学院华南植物园,广东广州510650;中国科学院大学,北京100049;2. 中国科学院华南植物园,广东广州,510650
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| 摘要: |
| 为研究功能基因在厚藤(Ipomoea pes-caprae L)中的表达和调控提供内参基因,本文根据Actin基因的保守区设计简并性引物,采用RT-PCR克隆厚藤的Actin基因片段,然后将获得的片段连接于克隆载体上进行测序,并运用分子生物学软件对该基因序列进行分析.结果表明,该基因片段大小为554 bp,编码184个氨基酸;该序列与其他植物Actin基因的cDNA序列的同源性均在80％以上,与氨基酸序列的同源性在94％以上.由此得出,本研究克隆的基因序列为Actin基因片段,将其命名为IpActin1,并登录在GenBank,登录号为KU564627. |
| 关键词: 厚藤 Actin基因 克隆 序列分析 |
| DOI： |
| 分类号:Q943.2 |
| 基金项目:中国科学院A类战略性先导科技专项(XDA13020500) |
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| Cloning and Sequence Analysis of Actin Gene Fragment from Ipomoea pes-caprae L |
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GUO Yan1,2,ZHANG Mei1,2,XIA Kuaifei1,2,JIAN Shuguang1,2,CHEN Jiantong1,2
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1.South China Botanical Garden,Chinese Academy of Sciences,2.University of Chinese Academy of Sciences
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| Abstract: |
| To provide housekeeping genes for studying the expression and regulation of functional genes in Ipomoea pes-caprae L,we designed a pair of degenerated primers and cloned the partial ActincDNA by RT-PCR,according to the conservative regions of other plant Actins.The cDNA fragments were cloned into T vector and sequenced.The results showed that this DNA fragment,including 554 bp,encoded a partial Actin with 184 amino acids.Homology comparison with other Actinsequences showed that it shared over 80%nucleotide sequence identity and 94%amino acid sequence identity with other Actins.This cDNA is named IpActin1,and is registered into GenBank(accession number:KU564627). |
| Key words: Ipomoea pes-caprae L Actin gene clone sequence analysis |
