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正交试验优化乙醇回流提取积雪草苷的工艺研究
李涛,朱慧娟
荆州市第一人民医院门诊药房,湖北荆州,434000
摘要:
为了优化积雪草中积雪草苷的提取工艺,本研究采用乙醇回流法,以积雪液草苷含量为研究指标,在单因素试验的基础上采用正交试验设计,考察乙醇浓度、液料比、粉碎粒度以及提取时间等因素对提取结果的影响.结果表明,积雪草苷的最佳提取工艺条件为:乙醇浓度70%、液料比10∶1、粉碎粒度140目、提取时间6h,该工艺条件下积雪草苷的提取含量达到8.32 mg·g-1.
关键词:  积雪草  积雪草苷  正交试验  提取工艺
DOI:
分类号:R284
基金项目:
Optimization of the Ethanol Reflux Extraction Process of Asiaticoside from Centella asiatica by Orthogonal Experiment
LI Tao,ZHU Huijuan
Outpatient Pharmacy,Jingzhou First People's Hospital
Abstract:
In order to optimize the extraction technology of the asiaticoside from the Centella asiatica,the orthogonal experiment was used to study the influence of ethanol concentration,solid-liquid ratio,particle size and extraction time on the basis of single factor test.The optimum extraction process was determined as follows:ethanol concentration was 70%,liquid-solid ratio was 10:1,grinding particle size was 140 mesh,extraction time was 6h.Under these conditions,the extraction content of asiaticoside reached to 8.32mg·g-1.
Key words:  Centella asiatica  asiaticoside  orthogonal experiment  extraction technology