| 摘要: |
| 以产L-缬氨酸的谷氨酸棒状杆菌(Corynebacterium glutamicum)为原始菌株,利用注入低能氮离子束进行一系列诱变,获得一株稳定的高产L-缬氨酸突变菌株.摇瓶培养96 h后发酵能力可达38.0g·L-1,较出发菌株提高18.01％.通过对摇瓶中葡萄糖、玉米浆浓度及培养条件进行优化,发酵能力达到40.6 g· L-1,50 L发酵罐的发酵能力可达70 g·L-1左右. |
| 关键词: L-缬氨酸 谷氨酸棒状杆菌 离子束 诱变育种 |
| DOI： |
| 分类号:TQ922 |
| 基金项目: |
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| Breeding of L-Valine High-Yielding Strain |
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XIE Fei,SHEN Lianbing,JIANG Bing
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Yichang Sanxia Pharmaceutical Co.,Ltd.
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| Abstract: |
| Using Corynebacterium glutamicum as the original strain for mutagenesis by low-energy ion beam implantation,a stable L-valine hyper-producing mutant strain was obtained.After 96 hfermentation in shaking flask,the concentration of L-valine reached to 38.0g·L-1,increased by 18.01%compared to the original strain.By optimizing the concentration of glucose and corn syrup and the culture conditions,the concentration of L-valine in shake flask was up to 40.6g·L-1,and the concentration of L-valine could reach to 70g·L-1in 50-liter fermentor. |
| Key words: L-valine Corynebacterium glutamicumion ion bean mutation breeding |
