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| 重组大肠杆菌高密度发酵生产RGD-TRAIL的条件优化 |
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曹家明1,韦利军1,袁建磊1,杭长标1,刘福松2
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1. 常州千红生化制药股份有限公司,江苏常州,213022;2. 中国药科大学生命科学与技术学院,江苏南京,210009
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| 摘要: |
| 本文以一株产RGD-TRAIL的重组大肠杆菌为研究对象,在10L发酵罐中考查了诱导温度、pH值、溶氧、流加葡萄糖对重组大肠杆菌生长和RGD-TRAIL蛋白表达的影响.结果表明:诱导温度25℃,pH值控制7.0,溶氧控制30％,以5 g·L-1·h-1流速流加葡萄糖最有利于菌体生长和蛋白表达,菌体收率和RGD-TRAIL产量分别达到45.99 g·L_1和160.2mg· L-1. |
| 关键词: RGD-TRAIL 重组大肠杆菌 发酵工艺 条件优化 |
| DOI： |
| 分类号:TQ920.6 |
| 基金项目:国家科技重大专项一重大新药创制(2009ZX09103-675;2012ZX09401012)，常州科技支撑计划(社会发展)(CE20115044) |
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| Optimization of Fermentation of High Density Recombinant E.coli for RGD-TRAIL |
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CAO Jiaming1,WEI Lijun1,YUAN Jianlei1,HANG Changbiao1,LIU Fusong2
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1.Changzhou Qianhong Bio-pharma Co.,Ltd.;2.School of Life Science and Technology,China Pharmaceutical University
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| Abstract: |
| To study and optimize the fermentation parameters for expressing RGD-TRAIL during recombinant E.coli high density fermentation,the effects of temperature,pH,dissolved oxygen,fed-batch of glucose on the cell growth and the protein expression were investigated to optimize the fermentation conditions.The results showed that the following conditions were best for the cell growth and the protein expression:temperature of 25℃,pH 7.0,dissolved oxygen of 30%,and glucose feeding rate at 5g·L~(-1)·h~(-1).Under the optimized conditions,the cell yield and RGD-TRAIL yield reached to 45.99g·L~(-1) and 160.2mg·L~(-1),respectively. |
| Key words: RGD-TRAIL recombinant E.coli fermentation optimization |
