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| 南海冷泉区深海沉积物中细菌的分离培养及多样性分析 |
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杨硕1,2,高峥1,2,邵宗泽1,3
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1. 山东农业大学生命科学学院,山东泰安271018;国家海洋局第三海洋研究所国家海洋局海洋生物遗传资源重点实验室,福建厦门361005;南海生物资源开发与利用协同创新中心,广东广州510300;2. 山东农业大学生命科学学院,山东泰安,271018;3. 国家海洋局第三海洋研究所国家海洋局海洋生物遗传资源重点实验室,福建厦门,361005
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| 摘要: |
| 为了认识南海深海冷泉区沉积物中可培养微生物的多样性,本文以冷泉区与非冷泉区两个站点的深海沉积物为样品,通过两种培养基(R2A海水培养基和2216 E培养基)直接涂布或富集后平板分离纯化,从9个样品中共得到395株菌株,并通过16SrRNA基因鉴定,分属10个属.发现产芽胞细菌分布最广、丰度最大,包括3个属、15个种.其中芽胞杆菌(Bacillus)无论是在数量还是在种类上都分布最多.并且,随着水深和沉积物深度的增加,分离到的可培养微生物丰富度降低.本研究表明,即使在冷泉区,南海深海沉积物中产芽胞细菌也比较丰富. |
| 关键词: 南海 冷泉区 深海沉积物 可培养微生物 产芽胞细菌 |
| DOI： |
| 分类号:Q939 |
| 基金项目:国家自然科学基金(41306150)，山东省优秀中青年科学家科研奖励基金(BS2012HZ011)，山东省高等学校科技计划项目(J10LC09)，国家海洋局海洋生物遗传资源重点实验室开放基金(HY201202)，中国大洋专项(DY125-15-R-01)，海洋公益性行业专项(201005032)，国家微生物资源平台项目(NIMR-2014-09) |
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| Diversity of Cultivable Bacteria in Deep Sea Sediments from Cold Seeps in the South China Sea |
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YANG Shuo1,2,GAO Zheng1,2,SHAO Zongze1,3
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1.College of Life Science,Shandong Agricultural University,Key Laboratory of Marine Genetic Resources,Third Institute of Oceanography,State Oceanic Administration,2.South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center
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| Abstract: |
| To investigate the diversity of the cultivable bacteria in deep sea sediments from cold seeps in the South China Sea,sediment samples collected from cold seeps and non-cold seeps were used to isolate microorganisms.395 bacterial strains were isolated on marine agar 2216 Eplates and marine R2 Aplates by direct spreading after sample dilution or plating after enrichment.The 16 SrRNA gene analysis showed that they belonged to 10 genera.Various spore-forming bacteria were most abundant,allocating to 15 species of 3genera,among which Bacillus was the dominant group.Moreover,the bacterial diversity decreased with the increase of sampling depth.These results indicate that the spore-forming bacteria from the deep sea sediments of South China Sea are widespread in high abundance,even in sediments nearby the cold seeps. |
| Key words: South China Sea cold seeps deep sea sediment cultivable microbes spore-forming bacteria |
