| 摘要: |
| 采用HPLC-ELSD法,Prevail C18柱,体积分数0.06%HFBA溶液-乙腈(88∶12)为流动相,柱温30℃,流速为0.8 ml·min-1,ELSD漂移管温度110℃,气体流量2.8 L·min-1,建立了测定L-鸟氨酸的方法.鸟氨酸在0.1～0.6 g·L-1范围内,线性回归比非常显著(r2=0.9998),平均回收率98.94%.该法简便、快速、准确性高、重复性好,适合于L-鸟氨酸的定量分析. |
| 关键词: L-鸟氨酸 高效液相色谱 ELSD |
| DOI： |
| 分类号:Q5 |
| 基金项目: |
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| Determination of L - Ornithine Content in Fermentation Broth by HPLC - ELSD |
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| Abstract: |
| Determination of L-ornithine content in the fermentation broth by HPLC-ELSD method was set up using Prevail C18 column.The mobile phase was 0.06%HFBA in water-acetonitrile(88∶12),with a flow rate of 0.8ml·min 1 at 30℃.The temperature of drift tube heater was 110℃and the gas was flowing at a rate of 2.8 L·min-1.A good linearity was obtained in the range of 0.1-0.6mg·ml-1(r2= 0.9998) for L-ornithine.The average recovery of L-ornithine was 98.94 %.The method is simple and rapid with reproducibility for the determination of L-ornithine in the fermentation broth. |
| Key words: L-ornithine HPLC ELSD |
