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从火棘果中提取精氨酸的研究
李玉洁,田萍,姚秉华
西安理工大学应用化学系,710054,西安
摘要:
以天然野生植物火棘果为原料分离提取精氨酸,考察了树脂类型、吸附方式、温度、pH值、洗脱液、脱色及精制方法等因素对提取精氨酸的影响,并对产物进行了分析鉴定.结果表明,在20℃、pH值7~8的条件下,选用D001型大孔阳离子交换树脂静态吸附,40 min即达到饱和;以3 mol·L-1氨水为洗脱液,流速控制在40 mL·h-1,精氨酸的洗脱率达到90%以上;采用711型阴离子树脂进行脱色,D001型大孔阳离子交换树脂动态精制,精氨酸的提取率达到95.83%.
关键词:  火棘果  精氨酸  提取  离子交换法
DOI:
分类号:Q50
基金项目:
Study on Extraction of Arginine the Pyracantha
Abstract:
Arginine was extracted from the natural wild plant Pyracantha.The resin type,absorption way,temperature,pH value,eluent,decolorization and purification were investigated.The result indicated: Using D001 macropore cation exchange resin at static adsorption,when the temperature was 20℃ and the pH value was between 7 and 8,the saturation could be achieved in 40min;The arginine eluation rate achieved above 90% when the eluent was 3mol·L-1 ammonia and the velocity of flow was 40mL·h-1;The product was decolored by 711 anion resin and purified dynamically by D001 macropore cation exchange resin,and the extraction rate was upto 95.83%.
Key words:  Pyracantha  arginine  extraction  ion exchange