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拟南芥耐盐突变体stg2的筛选
吕思敏
武汉大学生命科学学院,武汉,430072
摘要:
采取在高盐平板上萌发的方法,对一个雌激素诱导激活型拟南芥突变体库进行了耐盐突变体的筛选,最终得到了2株稳定的耐盐突变体.本文中对其中的一株耐盐突变体,命名为stg 2(salt tolerance during germination 2),进行了研究.遗传实验表明它的耐盐特性是受雌激素诱导的,是功能获得型的耐盐突变体.本实验中还探讨了stg2突变体的筛选过程及耐盐生理特点.
关键词:  拟南芥  诱导激活型突变体库  耐盐突变体
DOI:
分类号:Q94
基金项目:
Screening of Salt - Tolerant Mutant stg2 of Arabidopsis thaliana
Abstract:
By seed germinating on salt-containing media,a population of estrogen-inducible activation-tagging Arabidopsis thaliana mutant has been screened for salt-tolerant mutants,and two stable salt-tolerant mutants were obtained.One of them,designatedas stg2(salt tolerance during germination 2) was studied.Genetic analysis showed that its salt tolerance was induced by estrogen,thus it was a functional mutant.The isolation and characterization of stg2 mutant were also investigated.
Key words:  Arabidopsis thaliana  inducible activation-tagging mutant library  salt-tolerant mutant