| 摘要: |
| 里氏木霉(Trichoderma reesei)306是染色体上整合有组织型纤溶酶原激活剂(t-PA)cDNA的基因工程菌株.本论文利用筛选平板筛选出产t-PA活力高和产蛋白酶活力高的两种基因工程里氏木霉306菌株,然后对它们发酵过程中的产酶特性进行研究.结果表明:菌株产蛋白酶活力的大小与生物合成t-PA活力大小之间有一定的关系,产蛋白酶活力相对高的菌株产t-PA的活力相对低,而产蛋白酶活力相对低的菌株,产t-PA活力相对就高. |
| 关键词: 筛选 里氏木霉 组织型纤溶酶原激活剂(t-PA) |
| DOI： |
| 分类号:Q81 |
| 基金项目: |
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| Screening of Recombinant Trichoderma reesei 306 and Its Enzyme- Producing Property |
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| Abstract: |
| Trichoderma reesei 306 is a recombinant strain of which t-PA cDNA was recombined into its chromosome.Two kinds of recombinant Trichoderma reesei 306,namely t-PA-producing and proteinase-producing,were obtained.The result showed that the regularity was existed between proteinase activity and t-PA activity.The strain of higher proteinase activity produced lower t-PA activity and vice versa. |
| Key words: screening Trichoderma reesei tissue-type plasminogen activator(t-PA) |
