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| 利用反相高效液相色谱测定香椿叶中槲皮素含量 |
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李颖1,王昌禄1,刘常金1,夏廉法2,陈志强1,陈勉华1,江慎华1
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1. 天津科技大学食品工程与生物技术学院,天津市食品营养与安全重点实验室,天津,300457;2. 河南省农业科学院农产品加工研究所,河南,郑州,450002
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| 摘要: |
| 用乙醇对香椿叶粉末进行提取,树脂柱浓缩,真空冷冻干燥,甲醇溶解,制备香椿黄酮.用7.6 mol·L-1盐酸85℃水浴回流2 h进行水解,以反相ODS柱甲醇-水(体积比为50:50,磷酸调pH至2.53)为流动相,在波长368 nm处对香椿叶中的槲皮素进行分离、测定.结果表明:香椿叶中含槲皮素平均质量分数为1.28%,平均加样回收率为98.9%,RSD为1.75%,反相高效液相色谱法测定香椿叶中槲皮素含量操作简便易行、结果准确可靠. |
| 关键词: 香椿叶 槲皮素 反相高效液相色谱 |
| DOI： |
| 分类号:Q946.83+3 |
| 基金项目: |
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| Determination of Quercetin in Leaves of Toona sinensis by Reversed- Phase High Performance Liquid Chromatography |
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| Abstract: |
| A RP-HPLC method for the determination of quercetin in leaves of Toona sinensis was developed.Samples were mixed with HCl of 25%,which were heated at 85℃ for 2 hours.Quercetin was separated completely on an ODS column(4.6mm×250mm),using a mixture of methanol and water(50∶50,phosphoric acid made the pH 2.53) as mobile phase,with UV detection at 368nm.The results have shown that the content of quercetin was up to 1.28%,the recovery rate was 98.9% and RSD was 1.75%.The method is quick and simple with good accuracy and can be used for the quality control. |
| Key words: leaves of Toona sinensis quercetin reversed-phase high performance liquid chromatography |
