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| 魔芋种质资源的AFLP分析 |
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滕彩珠1,2,刁英1,2,易继碧3,宗刚琼4,陈永波4,韩永华4,胡中立1,2
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1. 武汉大学发育生物学教育部重点实验室,武汉,430072;广西师范大学生命科学学院,桂林,541004;2. 武汉大学发育生物学教育部重点实验室,武汉,430072;3. 湖北省恩施市白果中学,445037;4. 恩施州农科院魔芋研究所,恩施,445000
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| 摘要: |
| 利用AFLP技术对13份魔芋种质资源进行了DNA多态性分析.从10对引物中筛选出2对引物扩增基因组DNA,共获得112条带,其中64条为多态性标记,每个引物平均提供56个标记信息.UPMGA法聚类分析结果表明:魔芋资源的遗传多态性比较丰富,西盟魔芋和珠芽魔芋的关系比较近,白魔芋和花魔芋的关系比较近. |
| 关键词: 魔芋 AFLP 种质资源 多态性 |
| DOI： |
| 分类号:Q943 |
| 基金项目:湖北省科技攻关项目 |
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| AFLP Analysis of Relative Relationships Among Germplasm Resources of Amorphophallus |
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| Abstract: |
| The AFLP analysis was conducted in the 13 germplasm resources in Amorphophallus.Out of 10 pair primers 2 pair primers were selected and then amplified the 13 genomic DNAs.Total 112 AFLP fragments were generated,109 of them were polymorphic marks.Every primer offered 56 markers on average.The molecular phylogenetic tree obtained by UPMGA method showed genetic diversity was rich in Amorphophallus.Amorphophallus krausei is close to Amorphophallus guripingensis while Amorphophallus konjac is close to Amorphophallus albus. |
| Key words: Amorphophallus AFLP germplasm resources polymorphism |
