| 摘要: |
| 研究了无机与有机氮源对聚γ-谷氨酸合成与分泌的影响.分别在基础培养基及进入生物合成期的发酵液中添加氯化铵、硝酸钠和各种氨基酸,基础培养基中添加氯化铵质量浓度为1 g·L-1时,合成量提高了22.7%,同时,氯化铵对聚γ-谷氨酸组分也有一定影响.在培养至24 h添加8 g·L-1硝酸钠,合成量提高54.6%,而在基础培养基中分泌率提高了32.7%.在生物合成期添加8 g·L-1谷氨酸,合成量提高了64.2%.同时在静息细胞培养基中进行了验证实验. |
| 关键词: 氮源 聚γ-谷氨酸 合成 分泌 |
| DOI： |
| 分类号:TQ920 |
| 基金项目:山东省优秀中青年科学家科研奖励基金，山东省教育厅科研基金，曲阜师范大学校科研和教改项目 |
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| Influence of Nitrogen Sources on Synthesis and Secretion ofγ -Poly(glutamic Acid) |
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| Abstract: |
| The effects of inorganic and organic nitrogen sources on synthesis and secretion ofγ-Poly(glutamic acid) by flask fermentation were studied.NH_4Cl,NaNO_3 and various amino acids were added initially and in biosynthtic phase.The results showed that totalγ-Poly(glutamic acid) increased by 23% when the concentration of NH_4Cl was 1 g.L~(-1);component ofγ-Poly(glutamic acid) was also influenced by NH_4Cl;totalγ-Poly(glutamic acid) increased by 54.6 % when 8g.L~(-1) NaNO_3 was added at 24h;secretion ratio increased by 32.7 % when 8g.L~(-1) NaNO_3 was added intially;totalγ-Poly(glutamic acid) increased by 64.2 % when 8 g..L~(-1) glutamic acid was added in the biosynthetic phase. |
| Key words: nitrogen source γ-poly(glutamic acid) synthesis secretion |
