摘要: |
采用熏气法,可较为彻底地杀死魔芋球茎或根状茎的内生菌,再配合酒精、HgCl2等进行表面灭菌,可使初次接种污染率控制在10%以下,继代培养污染率控制在5%以下,从而建立魔芋试管苗的无菌繁殖体系,为试管苗的批量化生产提供了前提和技术保障. |
关键词: 魔芋 试管苗 消毒灭菌 批量 熏气法 |
DOI: |
分类号:O621 |
基金项目:湖北省西部少数民族科技开发项目 |
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Disinfection Technology for Young Konjac in Test Tube |
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Abstract: |
Endogenec bacteria in young koniec tube could be destroyed more completely by fumigating method, and the surface could be disinfected with alcohol and HgCl_2. The contaminative ratios was controlled under 10% on first inoculation, under 5% on the next inoculation. |
Key words: konjac young konjac tube disinfection large number of tubes fumigating method |