| 摘要: |
| 通过微量细胞病变抑制法,初步研究艾叶挥发油对呼吸道合胞病毒(RSV),流感病毒(IFV)的体外抑制作用。从艾叶挥发油的最大无毒量(TD0 )开始,作连续倍比稀释6个浓度,每一浓度均做4孔,分别加入已感染RSV的单层Hela细胞和感染IFV的单层MDCK细胞中,并设立阴性和阳性对照, 37℃,体积分数为5% CO2 培养,连续动态观察,得到药物的半数有效量(IC50 ),并计算治疗指数(TI)。艾叶挥发油对RSV的IC50为3. 33mg·L-1,TI值为9. 4。对IFV没有抑制作用。结论:艾叶挥发油对RSV有一定的抑制作用,对IFV的抑制作用不明显。 |
| 关键词: 艾叶挥发油 呼吸道合胞病毒 抗病毒作用 |
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| A Preliminary Study of Virus-Inhibiting Effect of Volatile Oil from Artemsia argyi |
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| Abstract: |
| The effect of volatile oil from Artemsia argyi on respiratory syncytial virus (RSV) and influenza virus (IFV) in vitro was studied. The antivirus effects on Hela and MDCK cell lines were observed by microcytopathic-inhibiting-test. Following the method of Reed-Muench assay, the effective dose of 50 percentages (IC 50) and treatment index (TI) were measured. The IC 50 and TI values to RSV were 3.33mg·L -1 and 9.4. There was no inhibiting effect on IFV. |
| Key words: Artemsia argyi volatile oil RSV antivirus effect |
