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产L-乳酸凝结芽孢杆菌发酵条件的初步研究
周剑,虞龙
南京工业大学制药与生命科学学院,江苏,南京,210009
摘要:
对经N+离子注入筛选到的L-乳酸高产菌株Bacillus coagulans RS12-6的发酵条件进行了初步研究.主要研究了通气量、温度及pH值等培养条件对菌体生长及产酸的影响.确定最佳培养条件及最佳碳源、氮源配比.
关键词:  L-乳酸  凝结芽孢杆菌  发酵条件优化
DOI:
分类号:TQ92
基金项目:江苏省高校自然科学基金
Study on L-Lactic Acid Fermentation of Bacillus coagulans
Abstract:
The preliminary research of cultivation conditions of the mutant strain RS12-6 obtained from N + implantation breeding has been done. The culturing conditions such as pH control, oxygen supply and temperature were studied deliberately. And the optimal carbon and nitrogen sources and their optimal concentration were determined through orthogonal design.
Key words:  L-lactic acid  Bacillus coagulans  fermentation