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优化黄姜中甾体皂甙元测定的研究
董静洲,易自力,蒋建雄
湖南农业大学细胞工程重点实验室,湖南,长沙,410128
摘要:
对传统的黄姜中甾体皂甙元的测定方法进行了改进.提取甾体皂甙元时采用微量样品以氯仿浸提代替传统的回流抽提法,以Liebemann-Barcharad试剂显色,用分光光度法测定.测定结果准确,高效低成本且步骤简单,应用广泛.
关键词:  黄姜  甾体皂甙元  微量浸提
DOI:
分类号:Q503
基金项目:
Improvement on Determination of Diosgenin in Dioscorea zingiberensis Wright
Abstract:
Improvement was made on the traditional way of determination of diosgenin. Diosgenin was extractied with chloroform instead of circumfluence and then oxidized by Liebemann-Barcharad reagent and finally determined with a spectrophotometer. This method was proved to be simple, accurate, low-cost and highly efficient.
Key words:  Dioscorea zingiberensis Wright  diosgenin  micro-extraction