| 摘要: |
| 研究了用快速分离柱高效液相色谱法测定红酵母细胞中β-胡萝卜素的方法.发酵法得到的样品经匀浆后用四氢呋喃提取,提取液以 ZORBAX Stable Bound (4.6×50 mm, 1.8μm) C18 快速分离柱为固定相,乙腈-四氢呋喃体积比 80:20为流动相分离,流速为 1.5 ml/min;在该色谱条件下,β-胡萝卜素在 1.0 min 内可达到基线分离;用紫外二极管矩阵检测器检测.方法标准回收率为98%～103%, 相对标准偏差为0.46%～0.58%,可用于几种发酵样品中β-胡萝卜素的测定,结果可靠. |
| 关键词: 高效液相色谱 β-胡萝卜素 红酵母 |
| DOI： |
| 分类号:Q56 |
| 基金项目:玉溪师范学院校科研和教改项目，云南省自然科学基金 |
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| Determination of β-Carotene in Red Yeast by Rapid Column High Performance Liquid Chromatography |
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| Abstract: |
| A rapid column high performance liquid chromatography (HPLC) method for determination of β-carotene in red yeast (Rhodotorula glutinis) was studied. The β-carotene was extracted from the sample with tetrahydrofuran by ultrasonic extraction. Then the β-carotene was separated on a ZORBAX Stable Bound (4.6×50 mm, 1.8μm) rapid chromatographic column with acetonitrile-tetrahydrofuran (80:20) as mobile phase at a flow-rate of 1.5 ml·min -1 , and monitored with the photodiode array detector at 451 nm. The recoveries of the β-carotene were 98% to 103%, and the relative standard deviations were 0.46% to 0.58%. This method was applied to the determination of β-carotene in red yeast with good results. |
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