| 摘要: |
| 用上海迪赛诺原L-谷氨酰胺生产菌种GB20为出发菌株,利用亚硝基胍诱变方法进行诱变筛选,获得目标株GB20-9090,再以目标株为发酵菌种,经过发酵过程的工艺优化,在5L小发酵罐生产水平达75 g·L-1以上,在70M3发酵罐生产水平达70 g·L-1以上. |
| 关键词: L-谷氨酰胺 亚硝基胍 工艺优化 |
| DOI： |
| 分类号:TQ92 |
| 基金项目: |
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| Breeding of L-Glutamine-Producing Strain and Optimization of Ifs Fermentation Process |
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| Abstract: |
| s: The original L-glutamine-producing strain GB20 was mutagenized by NTG, and the strain of GB20-9090 was obtained. The strain of GB20-9090 was fermented and got optimum conditions. The production level was 75g·L -1 of 5L fermeter and 70g·L -1 of 70M 3 fermeter. |
| Key words: L-glutamine NTG optimization |
