| 摘要: |
| 研究了初始葡萄糖浓度对D-核糖发酵的影响,证实了较高的葡萄糖浓度对D-核糖发酵的抑制作用,并确定了较为适宜的初始葡萄糖浓度为100 g·L-1或150 g·L-1 .前者条件下D-核糖的转化率和生产强度均达最大,分别为328g·kg-1和0.68 g·L-1·h-1;后者条件下D-核糖的产量达最大值39.48 g·L-1.针对底物抑制现象,研究了补料工艺对D-核糖发酵的影响,确定发酵24h后补加50 g·L-1的葡萄糖为较优的补料工艺,在此工艺条件下最终D-核糖产量相对于对照组提高了48.3%. |
| 关键词: D-核糖 葡萄糖 初始葡萄糖浓度 底物抑制 补料分批培养 |
| DOI： |
| 分类号:TQ923 |
| 基金项目:河北省自然科学基金 |
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| Study of Substrate Inhibition and Fed Batch Culture in The Fermentation of D-Ribose |
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| Abstract: |
| The effects of initial glucose concentration on D-ribose fermentation were studied. The inhibiting effects of higher glucose concentration on D-ribose fermentation were proven. Appropriate initial glucose concentration, 10% or 15% was obtained. Under the former condition the maximal D-ribose yield and productivity, 0.328g·g - and 0.068g·100ml -·h -, were reached respectively. Under the latter condition the maximal D-ribose concentration, 3.948g·100ml -, was reached. In order to avoid substrate inhibition fed-batch culture was studied. Adding 5% glucose after 24h fermentation was established as the appropriate method to increase the D-ribose concentration. With this fed-batch culture method the ultimate D-ribose concentration was 48.3% higher than that of the control. |
| Key words: D-ribose glucose initial glucose concentration substrate inhibition fed batch culture |
