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| 血清游离精氨酸的快速检测 |
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孙玮1,2,路鞠1,2,付晓岚1,2,苟俊1,2,尤忠义1,2
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1. 第三军医大学,中心实验室,重庆,400038;2. 第三军医大学,附属西南医,院烧伤研究所,重庆,400038
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| 摘要: |
| 建立快速、准确的精氨酸定量检测方法.采用6300黄金系统氨基酸分析仪,在锂柱生理体液分析方法基础上,建立血清游离精氨酸(ARG)快速测定方法.血清样本经磺基水杨酸沉淀蛋白后取上清液进行色谱分析,色谱柱为Beckman公司阳离子交换柱(12cm ×4.0 mm);流动相为20 mmol*L-1柠檬酸锂水溶液,流速为20 ml*h-1;比色波长570nm.该法检测精氨酸浓度的线性范围为5mg*L-1～50 mg*L-1,相关系数0.99834,最低检测限1 mg*L-1,重复性:日内RSD 0.40%,日间RSD 0.55 % ,回收率97.67%～100.67%(平均值99.07%);整个实验过程耗时28min.该法简便、快速、准确、可靠,适用于临床和科研工作. |
| 关键词: 精氨酸 氨基酸分析仪 快速检测 血清 |
| DOI： |
| 分类号:Q503 |
| 基金项目: |
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| The Method of Rapid Determination of Serum Arginine |
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| Abstract: |
| A rapid,precise and reliable method for detecting serum argininie was established.The physiological fluid was developed with 6300 system gold high performance amino acid analyzer. Serum sample was precipitated by 10 % sulfosalicylic acid and centrifuged to remove protein and then assayed.The operation conditions were ion-exchange column(12cm×4.0mm),20mmol.L -1 lithium citrate as the mobile phase at a flow rate of 20.0 mL/h.The detector was operated at λ570nm . The method was proved to be linear in the range of 5mg.L -1～50mg.L -1 with a regression coefficient of 0.99834. The minimun detection limit was 1 mg.L -1.The recoveries were 97.67% ～ 100.67%,the average was 99.07%.The detection time of serum Arg was within 28 min after injection. |
| Key words: argininie amino acid analyzer rapid determination serum |
