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用高效液相色谱法(HPLC)测定肌苷中有关物质和降解产物
刘林,陈蔚江,李金屏
湖北省药品检验所,湖北,武汉,430064
摘要:
用HPLC测定肌苷中有关物质和降解产物,与主药有良好的分离效果.色谱柱为Hypersil C18(250mm×4.6mm,5μm),流动相甲醇-水(甲醇与水体积比为10∶90),流速1mL*min-1,检测波长248nm.
关键词:  肌苷  高效液相色谱法  有关物质
DOI:
分类号:Q503
基金项目:
HPLC Determination of Related Compounds of Isonine
Abstract:
Related compounds of isonine were determinated by HPLC .The analytical column was ODS C18(length:250mm×4.6mm;filler:Hypersil;size:5μm).The mobile phase consisted of methanol and water(10:90)and the flow rate was 1ml.min -1 . The detection was carried out with UV detector and the detection wavelength of 248nm was specified.The proposed method was selective,simple and accurate.
Key words:  related compounds  isonine  HPLC