| 摘要: |
| 在0.10 mol/mL的醋酸溶液中, 4,5-二溴荧光素能与血清蛋白形成稳定的复合物,最大吸收波长482 nm,与试剂比较,红移了12 nm.据此建立了测定血清蛋白的方法,用于BSA和HSA的测定,分别在2～14 mg*L-1有线性关系,表观摩尔吸光系数分别为3.12×105 L*mol-1*cm-1和3.27×105 L*mol-1*cm-1.应用该法测定了人血清样品总蛋白含量,结果令人满意. |
| 关键词: 4,5-二溴荧光素 牛血清白蛋白(BSA) 人血清白蛋白(HSA) 分光光度法 |
| DOI： |
| 分类号:Q67 |
| 基金项目:湖北省自然科学基金，中南民族大学校科研和教改项目 |
|
| Study on Interaction between Serum Protein and 4,5-Dibromofluorescein and Its Application |
|
|
| Abstract: |
| In 0.10 mol/L acetic acid, 4,5-dibromofluorescein could combine with BSA or HSA to form a stable complex, which presented a maxium absorption at 482nm with 12nm of red shift compared to the reagent itself. The linear range for serum protein determination was 2～14μg/ml and the molar absorbtivity of 4,5-dibromofluorescein-BSA complex was 3.12×105 Lmol -1·cm -1 and of 4,5-dibromofluorescein-HSA complex 3.27×105 Lmol -1·cm -1. This method has been used to detect the total proteins of serum sample with satisfactory results. |
| Key words: dibromofluorescein bovine serum albumin (BSA) human serum albumin (HSA) spectrophotometry |
