| 本文已被：浏览 1489次 下载 0次 |
码上扫一扫！ |
|
|
| 柴胡提取物对小鼠的体外免疫效应 |
|
郭明雄1,2,孙桂鸿1,2,张文仁3,白惠卿3,冯胜彦1,2,陈蔚梅1,2
|
|
1. 武汉大学生命科学学院,湖北武汉,430072;2. 武汉大学生命科学学院,湖北武汉,430072;武汉大学医学院,湖北武汉,430071;3. 北京大学医学部免疫学系,北京,100083
|
|
| 摘要: |
| 利用淋巴细胞转化实验,肿瘤坏死因子α和白细胞介素2的诱生和活性检测的方法,观察南、北柴胡提取物在体外对正常小鼠的免疫功能影响.结果显示:1.北柴胡BuOH-H2O水溶物、北柴胡50%Eton提取后药渣组分、北柴胡50%Eton提取物以及南柴胡水提物能增强淋巴细胞转化作用;2.不同柴胡提取物对IL-2的产生均有显著增强作用;3.北柴胡BuOH-H2O水溶物、北柴胡50%Eton提取物以及南柴胡水提物能显著增强TNFα的产生.本实验表明,南、北柴胡提取物能增强机体的细胞免疫功能,但南、北柴胡不同提取物的免疫作用有差异. |
| 关键词: 柴胡提取物 淋巴细胞转化 白细胞介素2 肿瘤坏死因子α |
| DOI： |
| 分类号:Q956 |
| 基金项目: |
|
| Effect of Extracts of Bupleurum on In Vitro Immunity of Mice |
|
|
| Abstract: |
| This experiment was carried out by assaying the lymphocyte transformation ability, the induced level of interleukin-2(IL-2) and tumor necrosis factor α (TNFα) to observe the effect of southern Bupleurum (SB) and Bupleurum chinese DC(BCDC) on the immunity of normal mice in vitro. The results indicatel; 1.The BuOH-H 2O soluble part of Bupleurum chinese DC, the 50% Eton's extract of Bupleurum chinese DC and the aqueous extract of southern Bupleurum could enhance the lymphocyte transformation ability; 2.All kinds of extracts of Bupleurum had increasing effects on the induced level of IL2; 3.The BuOH-H 2O soluble part of Bupleurum chinese DC, the 50% Eton's extract of Bupleurum chinese DC and the aqueous extract of southern Bupleurum had increasing effects on the induced level of TNFα. |
| Key words: extract of Bupleurum lymphocyte transformation interleukin-2 tumor necrosis factor α |
