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| 鲎试剂在抗淋巴细胞免疫球蛋白热原检测中的应用 |
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艾智武1,2,叶林柏1,2,康光明1,3
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1. 武汉大学生命科学学院,武汉,430072;武汉生物制品研究所,武汉,430060;2. 武汉大学生命科学学院,武汉,430072;3. 武汉生物制品研究所,武汉,430060
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| 摘要: |
| 报道了鲎试剂在抗淋巴细胞免疫球蛋白内毒素检查中的应用.在用鲎试剂检测细菌内毒素时,按中国药典的要求对鲎试剂灵敏度进行标定,并作干扰实验(增强或抑制实验),然后用鲎试法与家兔法同时测定15批抗淋巴细胞免疫球蛋白.结果发现三批鲎试剂的灵敏度标示值均正确,五批抗淋巴细胞免疫球蛋白半成品在最大有效稀释度(MVD)时对试验无干扰,这两种方法测定结果的符合率达到91.7%.从而表明鲎试法很有可能代替家兔法. |
| 关键词: 抗淋巴细胞免疫球蛋白 鲎试剂 家兔 符合率 |
| DOI： |
| 分类号:R331.44 |
| 基金项目: |
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| Application of Limulus Amoebocyte Lysate Reagent to Estimating Pyrogen in Antilymphocyte Immunoglobulin |
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| Abstract: |
| The application of Limulus Amoebocyte Lysate(LAL) reagent to estimating pyrogen endotoxins in antilymphocyte immunoglobulin(ALG) was investigated. The LAL reagent sensitivity was confirmed and the interference test(enhancement or inhibition test) was done before using the reagent for examining the concentration of the bacterial endotoxins. 15 lots of ALG semi-finished productswere determined with LAL test and rabbit test. The result showed that the labeled values of three LAL reagent sensitivity were right and there was no interference to the test in five ALG diluted to Maximum Valid Dilution(MVD). The coincidence rate of two tests was 91.7%. This indicated that rabbit test may be replaced by LAL test. |
| Key words: ALG LAL reagent Rabbit test coincidence rate |
