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pH值对D-核糖发酵的影响及补料发酵的研究
郭建锋,顾晓波,王昌禄,祝嫦巍,唐如星
天津轻工业学院,天津,300222
摘要:
研究了不同pH值对D-核糖产量的影响.发酵初期pH自然下降时有利于菌体生长,菌体生长对数期较长,菌体质量浓度最高可达15.3 g/L;发酵中后期pH值控制在7.0时有利于D-核糖的持续合成,同时对D-核糖的流加补料发酵进行了初步研究,最终使菌体质量浓度最高达到20.1 g/L,D-核糖产量达到了62.5 g/L.
关键词:  D-核糖  pH控制  补料发酵
DOI:
分类号:Q935
基金项目:天津市自然科学基金
Effect of Different pH on Fermentation of D-Ribose and Fed Batch Cultivation
Abstract:
The effect of different pH on biomass and D-ribose production during the process of batch fermentation was tested. It was showed that cultivation of the strain without controlled pH was advantageous to obtain high level of dry cell weight and lengthen the logarithmic phase. The period of D-ribose production was prolonged when pH of the medium was set at 7.0 during the stationary phase. Fed batch fermentation of D-ribose was preliminarily studied,and the dry cell weight and the yield of D-ribose reached 20.lg/L and 62.5g/L respectively.
Key words:  D-ribose  pH control  fed batch fermentation