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高效液相色谱法分离测定大鼠肝线粒体膜磷脂
姜秋芬,骆子生,李兴发,姜玲玲
河北医科大学基础医学研究所 石家庄 050017
摘要:
建立了HPLC方法、运用梯度洗脱同时分离测定大鼠肝线粒体膜中的心磷脂(C)、磷脂酰肌醇(PI)磷脂酰乙醇胺(PE)、磷脂酰胆硷(PC)、鞘磷脂(SM)。结果表明,方法的线性关系、精密度、重现性较好。
关键词:  HPLC  线粒体膜  磷脂
DOI:
分类号:Q682
基金项目:
Determination of phospholipid in the mitochondial membrane of rat liver with HPLC
Abstract:
A method was developed for the determination ofcardiolipin,phosphatidyllinositol,phosphatidylserine,phosphatidylethanolamine,phosphatidylcholine and sphingomyelin in the mitochondrial membrane of rat liver with HPLC.The seperation was performed by gradient elution. The result indicated that the method was satisfying in its linear relationship, reproducibility and stability.
Key words:  HPLC, Mitochondria membrane, Phospholipids.