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| 乳酸抗性酵母的筛选及其生长特性的研究 |
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王昌禄1,2,杜连祥1,2,顾晓波1,2,路福平1,2,赵学明1,3,饭村( )穰4,冈崎直人4
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1. 天津轻工业学院,天津,300222;天津大学化工学院,天津,300071;2. 天津轻工业学院,天津,300222;3. 天津大学化工学院,天津,300071;4. 国税厅酿造研究所,日本国东广岛市,739
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| 摘要: |
| 以酿酒酵母(saccharomyces ceevisiae)单倍体YNN-27(α t ep-ura-)为亲株,在含有4%乳酸的梯度平板上直接进行紫外线诱变处理,筛选到突变株 YNN-27-24.通过对该突变株乳酸抗性产生原因分析、在含有不同浓度的乳酸和潮霉素B(hyg romycin B)的YPDL和YPDLH培养基中的重复特性的研究发现,该突变株对乳酸和潮霉素B产生 的抗性,不是因对环境条件的适应而产生,而是由基因突变所引起.与突变株YNN27-24相比 ,乳酸对亲株生长的影响在于延长了其生长的延迟期,而其生长速率没有发生改变.用Mini -photo 518测定供试菌株在生长过程中的吸光度(660 nm)以研究酵母菌的生长特性,是一种行之有效的方法,具有较高的灵敏度和较好的再现性. |
| 关键词: 酿酒酵母 乳酸抗性 潮霉素B |
| DOI： |
| 分类号:Q935 |
| 基金项目:中国-日本合作项目 |
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| Isolation and Characterization of Yeast Conditional Mutants with Lactic Acid Tolerance |
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| Abstract: |
| This paper mainly deals with the breeding of yeast mutants with lactic acid tolerance isolated on YPDL Containing 4% of lactic acid gradient agar plate and with the character analysis.It was observed that lactic acid tolerance of the strain was not an adaptation to the environment,but a real gene mutation.Compared with the growth characters of wild type YNN-27 in YPDL and YPELH medium,the mutant YNN-27-24 only shortened the lag phase and its growth rate did not change in the same medium. |
| Key words: Saccharomyces cerevisiae Lactic Acid tolerance Hygromycin B |
