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胶原海绵的羟脯氨酸含量测定
关静,叶萍,武继民
军事医学科学院卫生装备研究所,天津,300161
摘要:
选用普通实验室均能实现的Woessner第Ⅰ法对自制胶原海绵和Gelfix(国外样品)进行了羟脯氨酸含量测定,结果表明,该方法操作简单,重复性好:自制胶原海绵的羟脯氨酸含量稳定,与国外样品的羟脯氨酸含量接近,且与胶原蛋白的羟脯氨酸含量接近,证实了两种胶原海绵的纯度均较高.
关键词:  胶原海绵  羟脯氨酸  含量测定
DOI:
分类号:Q517
基金项目:
The determination of hydroxyproline in collagen sponge
Abstract:
The quantitative determination of hydroxyproline in collagen sponge, prepared by ourselves and Gelfix (abroad sample), was carried out by means of Woessner method Ⅰ. It showed that Woessner method Ⅰ was simple and the result was reprcduceable. The content of hydroxyproline in collagen sponge prepared by ourselves was stable and similar to that in Gelfix, and was very similar to that of the collagen protein, therefore the high purity of both kinds of collagen sponge was confirmed.
Key words:  collagen sponge  hydroxyproline  content assay