| 摘要: |
| 药用植物有效成分的积累不仅受遗传调控，更与其体内复杂的微生物密切相关。然而，玉竹作为重要中药材，其微生物组与品质形成的关系尚未明确。本研究采用Illumina Miseq测序技术对玉竹根茎不同时期内生细菌群落进行序列测定，并使用PICRUSt2软件进行16S功能预测分析。测定其有效成分类黄酮、皂苷和多糖的动态变化，筛选与玉竹关键次生代谢产物显著相关的内生细菌群落。通过OTUs序列分析发现假单胞菌属(Pseudomonas)是苗期的优势菌属，无色杆菌属(Achromobacter)是花期、果期和枯萎期优势菌属；PCoA分析结果表明玉竹内生细菌群落存在阶段性差异；通过LEfSe分析确定假单胞菌属、α-变形菌(Alphaproteobacteria)、绿脓杆菌(Pseudomonas aeruginosa)等为玉竹不同生长时期的生物标识物；功能预测结果显示，代谢途径被确定为所有样品中的主要功能；相关性分析的结果表明不同时期存在差异的前15个关键OTUs与黄酮、皂苷、多糖含量呈显著相关。本研究首次系统地揭示了栽培玉竹内生细菌的演替规律及其与药效成分累积的密切关联，不仅为解析玉竹品质形成的微生物学机制提供了关键证据，也为后续开发靶向微生物菌剂以提升药材品质奠定了理论基础。 |
| 关键词: 玉竹 内生细菌 群落多样性 高通量测序 次生代谢产物 |
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| Diversity of Endophytic Bacteria in Cultivated?Polygonatum odoratum?and Its Correlation with Quality Components |
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FENG Jie, WEI Yan
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School of Pharmacy,Heilongjiang University of Chinese Medicine
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| Abstract: |
| The accumulation of bioactive compounds in medicinal plants is not only genetically regulated but also closely linked to their complex microbiomes. However, the relationship between the microbiome and quality formation in Polygonatum odoratum, an important Chinese medicinal herb, remains unclear. This study employed Illumina MiSeq sequencing technology to determine the sequence the endophytic bacterial communities in the rhizomes at various periods. Illumina Miseq sequencing technology was employed to sequence the endophytic bacterial communities in the rhizomes at various periods. Functional prediction of 16S rRNA genes was performed using PICRUSt2 software. The dynamic changes in the active components, including flavonoids, saponins, and polysaccharides, were measured, and endophytic bacterial communities significantly associated with key secondary metabolites of P. odoratum were identified.OTU analysis revealed that Pseudomonas was the dominant genus at the seedling stage, while Achromobacter was dominant at the flowering, fruiting, and withering stages. PCoA results indicated stage-specific differences in the endophytic bacterial communities of P. odoratum. LEfSe analysis identified Pseudomonas, Alphaproteobacteria, and Pseudomonas aeruginosa as biomarkers at different growth stages. Functional prediction results showed that metabolic pathways were the predominant functions across all samples.Correlation analysis demonstrated that the top 15 key OTUs exhibiting temporal variation are significantly correlated with flavonoids, saponins, and polysaccharides content. This study systematically reveals, for the first time, the succession patterns of endophytic bacteria in cultivated P.odoratum and their close association with the accumulation of pharmacologically active components. It not only provides key evidence for elucidating the microbial mechanisms underlying the quality formation of P. odoratum, but also lays a theoretical foundation for the subsequent development of targeted microbial inoculants to improve the quality of medicinal materials. |
| Key words: Polygonatum odoratum (Mill.) Druce endophytic bacteria community diversity analysis high-throughput sequencing secondary metabolites |
