| 摘要: |
| 以新型物质D-茶氨酸的酶法制备过程为研究对象，考察了HZ201（Cl-）强碱型阴离子树脂在不同温度下吸附情况及动态实验的洗脱流速，研究结果表明：在25 ℃时，以8 mL/min稀HCl洗脱，分离出D，L-N-乙基海因丙酰胺酶转化产物，经结构鉴定为N-氨甲酰-D-茶氨酸，该新物质收率达71%，为D-茶氨酸的生物法制备提供了新途径。 |
| 关键词: 碱性树脂 N-氨甲酰-D-茶氨酸 分离 |
| DOI：10.14188/j.ajsh.2018.04.010 |
| 分类号:TQ 028.8 |
| 基金项目: |
|
| Application of HZ201 resin in enzymatic preparation of N-carbamoyl-D-theanine |
|
ZHANG Furong
|
|
Jiangsu Shenhua Pharmaceutical Co.， Ltd， Huai’an 211600， Jiangsu， China
|
| Abstract: |
| For enzymatic preparation of the new substance D-theanine， adsorption conditions at different temperatures and elution flow rate of the basic resin HZ201 were studied. The result showed that enzymatic transformation product from N-ethyl-3-（2，5-dioxoimidazolidin-4-yl） propanamide was separated by gradient elution of flow rate 8 mL/min with dilute hydrochloric acid at 25 ℃， which was identified as N-carbamoyl-D-theanine with the yield of 71%， providing a new way for the biological preparation of D-theanine. |
| Key words: basic resin N-carbamoyl-D-theanine separation |
