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| 摘要: |
| 考察平板计数琼脂(PCA)中不同来源的酵母浸粉对6种细菌总数测定的影响,并对培养基中酵母浸粉和胰蛋白胨的配比及含量进行了优化。优化后的培养基组分为胰蛋白胨3.0 g·L-1,酵母浸粉3.0 g·L-1,葡萄糖1.0 g·L-1,琼脂15.0 g·L-1。与市售平板计数琼脂相比,能检出的细菌总数提高6.9%。 |
| 关键词: 细菌总数 培养基优化 酵母浸粉 胰蛋白胨 |
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| Optimization of Medium for the Determination of Total Count for Six Bacteria |
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| Abstract: |
| The effects of different yeast extracts on the determination of total bacterial count were investigated for 6 bacteria. The proportion and content of yeast extract and tryptone were also optimized. The optimal medium contained tryptone 3.0 g·L-1,yeast extract 3.0 g·L-1,glucose 1.0 g·L-1,agar 15.0 g·L-1. The total bacterial count of optimal medium would be 6.9% higher than PCA. |
| Key words: total bacterial count medium optimization yeast extract tryptone |
