| 摘要: |
| 以E.coli JM83染色体DNA为模板PCR扩增ubiA的结构基因,将PCR产物克隆于pUCm-T Vector,对PCR产物进行测序鉴定.与已发表的大肠杆菌ubiA基因序列比较,同源性达99%. |
| 关键词: 辅酶Q10 光合细菌 ubiA 克隆 |
| DOI： |
| 分类号:Q78 |
| 基金项目: |
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| Cloning and Analysis of DNA Sequence of Gene ubiA of E.coli |
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| Abstract: |
| A DNA fragment was amplified from the chromosomal DNA of E. coli JM83 by PCR and then was cloned into pUCm - T vector. The cloned fragment was sequenced. Comparing the DNA sequence of the amplified fragment with the published sequence of gene ubiA. showed that they were 99% homologous. |
| Key words: ubiquinone - 10 photosynthetic bacteria ubiA cloning |
