| 摘要: |
| 通过对食品安全级(GRAS)乳酸菌的筛选,得到一株可高产谷氨酸脱羧酶的菌株.并且对菌株的发酵培养基与发酵条件进行了优化.结果表明:采用优化后的培养基,调初始pH为7.0,在33℃进行发酵培养3d后,发酵液中GABA含量可达到310g·L-1以上,比优化前提高了约4倍. |
| 关键词: 谷氨酸脱羧酶 γ-氨基丁酸 乳酸菌 发酵 |
| DOI: |
| 分类号:TQ921 |
| 基金项目: |
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| Studies on Screening for γ-Amino Butyric Acid Producer and Optimization of Fermentation Conditions |
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| Abstract: |
| A GRAS lactic acid bacterium was screened for its high glutamate decarboxylase production. The fermentation conditions were optimized. The result showed that the content of GABA was increased about 4 times. It was over 310mg·ml - in the optimum culture after fermentation for 3 days at 33℃ and with the beginning pH at 7.0. |
| Key words: glutamate decarboxylase(GAD) γ-amino butyric acid(GABA) lactic acid bacterium fermentation |