| 摘要: |
| 应用"化学比色法"对发酵液中L-缬氨酸进行了定量分析研究,确立了L -缬氨酸定量测定条件和计算方法.以高速氨基酸分析仪测定结果为参比,用化学比色法测定结果的准确度比纸层析-色斑洗脱比色法高,且应用化学比色法测定L-缬氨酸的含量较为方便. |
| 关键词: 缬氨酸 化学比色法 |
| DOI: |
| 分类号:R12 |
| 基金项目: |
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| Study on Chemical Colorimetry Determining L-valine in Fermented Broth |
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| Abstract: |
| L-valine concentration in fermented broth was quantitatively determined with methods of chemical colorimetry. The quantitative determination conditions and computing method were established. Compared with high-speed amino acid analyzer, the determination value of L-valine concentration with chemical colorimetry was more accurate and convenient than with paper chromatography-mottle elution colorimetry. |
| Key words: L-valine chemical colorimetry |