| 摘要: |
| 这是现代生物技术在环境微生物学中的应用系列综述文章的第二篇,讨论聚合酶链反应.这篇文章包含PCR步骤,特异基因的PCR检出,逆转录酶PCR,综合细胞培养PCR,多重PCR和半嵌套式PCR和PCR指纹法. |
| 关键词: 现代生物技术 环境微生物学 聚合酶链反应 |
| DOI： |
| 分类号:Q9306 |
| 基金项目: |
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| Application of Modern Biotechnology to Environmental Microbiology:Ⅱ.Polymerase Chain Reaction |
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| Abstract: |
| This is the second review in a series papers of application of modern biotechnology to evironmental microbiology.It deals with polymerase chain reaction and contains the steps of PCR, PCR detection of a specific gene, RT-PCR, ICC-PCR,multiplex and seminested PCR and PCR fingerprinting. |
| Key words: modern biotechnology environmental microbiology polymerase chain reaction |
